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NF-κB Pathway Inhibition (Primary Mechanism) KPV’s most well-characterized mechanism is inhibition of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells), the master regulator of inflammation: Cellular Entry via PepT1: KPV enters inflamed cells through PepT1 transporter (oligopeptide transporter 1) Nuclear Translocation Block: Prevents NF-κB translocation from cytoplasm to nucleus Transcription Inhibition: Blocks NF-κB-mediated transcription of pro-inflammatory genes Cytokine Reduction: Decreases IL-1β, IL-6, TNF-α, IL-8 production 2